![Fplc principles and procedures Fplc principles and procedures](/uploads/1/1/9/8/119877514/510571948.jpg)
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Fplc Principles And Procedures
Hello all,
System is run by a LCC-501 and contains two P-500 pumps. The detector is a UV-M II. FRAC-100 fraction collector. REC-102 chart recorder. The instrument is located in a 4 C cold room.
On my chromatograms, I am getting a periodic spike up and down and I cannot seem to locate the issue. I have removed the column and re-tighten all the positions and flushed liters of water.
I checked the flow by running a program for 10 mL at 0.25 mL/min into a graduated falcon 15 mL tube and the final volume was right on the mark.
The chart scale also seems to be at rate of 0.8 of what it should be. 10 mL at 0.1 cm/mL comes out to about 0.8 cm. The manual mentions calibrating the pulses from the pumps but does not mention how to do so.
If anyone is familiar with this instrument and has any ideas for either of these problems I would appreciate the insight.
Thank you.
System is run by a LCC-501 and contains two P-500 pumps. The detector is a UV-M II. FRAC-100 fraction collector. REC-102 chart recorder. The instrument is located in a 4 C cold room.
On my chromatograms, I am getting a periodic spike up and down and I cannot seem to locate the issue. I have removed the column and re-tighten all the positions and flushed liters of water.
I checked the flow by running a program for 10 mL at 0.25 mL/min into a graduated falcon 15 mL tube and the final volume was right on the mark.
The chart scale also seems to be at rate of 0.8 of what it should be. 10 mL at 0.1 cm/mL comes out to about 0.8 cm. The manual mentions calibrating the pulses from the pumps but does not mention how to do so.
If anyone is familiar with this instrument and has any ideas for either of these problems I would appreciate the insight.
Thank you.